Human CellExp™ CD74/DHLAG, human recombinant

Biovision

Size

50 μg

Catalog No.

7387-50

Price

719 EUR

Background

Cluster of Differentiation 74 (CD74) also known as HLA class II histocompatibility antigen gamma chain (HLADG), is a polypeptide involved in the formation and transport of MHC class II protein. The cell surface form of the invariant chain is known as CD74. CD74 is upregulated in several cancers and is also expressed by non-immune cells during inflammation. CD74 plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha / beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal / lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. CD74 / DHLAG also serves as cell surface receptor for the cytokine MIF.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

How to reconstitute

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Molecular weight

This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 19.3 kDa. The predicted N-terminus is Gln 73. DTT-reduced Protein migrates as 23-33 kDa due to glycosylation.

Biological activity

Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/ml (100 μl/well) can bind biotinylated human CTSL1 with a linear range of 3.2 - 500 ng/ml.

Reesults

Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/ml (100 μl/well) can bind biotinylated human CTSL1 with a linear range of 3.2 - 500 ng/ml.

Physical properties

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Synonym

CD74, DHLAG, HLADG, II, Ia-GAMMA, p33, CLIP, INVG34

Other name

CD74, DHLAG, HLADG, II, Ia-GAMMA, p33, CLIP, INVG34

Notes

For Research Use Only! Not to be used in humans

Before use

Centrifuge the vial prior to opening.

Additional source

Recombinants or rec. proteins

Tested applications

SDS-PAGE, N/A

Group

recombinants

Source

HEK293 cells

Physical appearance

Lyophilized

Target

CD74/DHLAG

Shipping under

Gel Pack

Purification

≥95%

Storage condition

-20°C

NCBI number

P04233

Gene source

Human

NCBI gene

CD74

Tested activity

N/A

Binding ability

N/A

Unit

N/A

Recombinant

Yes

Aa sequence

N/A

NCBI gene number

972

Concentration

N/A