Recombinant Human HDAC4 Protein (aa 612-1084)

Elabscience

Size

20µg

Catalog No.

PKSH030998-20µg

Price

356 EUR

Background

HDAC4 (histone deacetylase 4), belongs to class II of the histone deacetylase/acuc/apha family. Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general, they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions and by the class to which they belong. HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents. HDAC4 possesses histone deacetylase activity and represses transcription when tethered to a promoter. It does not bind DNA directly, but through transcription factors MEF2C and MEF2D. HDAC4 seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability and Storage

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Formulation

Lyophilized from sterile 20mM Tris,500mM NaCl, pH 7.4, 10%gly

Endotoxin

<1.0 EU per µg as determined by the LAL method

Synonym

AHO3;BDMR;HA6116;HD4;HDAC-4;HDAC-A;HDACA

Purity

>90 % as determined by SDS-PAGE

Source

Recombinants or rec. proteins

Expressed Host

Baculovirus-Insect Cells

Group

recombinants

Accession

NP_006028.2

Sequence

Gly & Pro

Mol Mass

50.9 kDa

AP Mol Mass

51 kDa

Activity

NA

Fusion tag

NA