Recombinant Human Histone Chaperone ASF1A/ASF1A (C-6His, N-T7 tag)
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Reconstitution conditions
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage conditions
Lyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at below -20°C for 3 months.
Peptide sequence
MASMTGGQQMGRGSMAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCMLEHHHHHH
Description
Recombinant Human Histone Chaperone ASF1A is produced by our E.coli expression system and the target gene encoding Met1-Met204 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.
Package form
Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl, 1mM DTT, 150mM NaCl, pH 8.0.
Endotoxin level
Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Protein purity
Greater than 95% as determined by reducing SDS-PAGE.
Source
Recombinants or rec. proteins
Shipping condition
Ambient/Room Temperature
Estimated molecular weight
25,4 kDa
