Human CellExp™ CD74/DHLAG, human recombinant
Background
Cluster of Differentiation 74 (CD74) also known as HLA class II histocompatibility antigen gamma chain (HLADG), is a polypeptide involved in the formation and transport of MHC class II protein. The cell surface form of the invariant chain is known as CD74. CD74 is upregulated in several cancers and is also expressed by non-immune cells during inflammation. CD74 plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha / beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal / lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. CD74 / DHLAG also serves as cell surface receptor for the cytokine MIF.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Molecular weight
This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 19.3 kDa. The predicted N-terminus is Gln 73. DTT-reduced Protein migrates as 23-33 kDa due to glycosylation.
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/ml (100 μl/well) can bind biotinylated human CTSL1 with a linear range of 3.2 - 500 ng/ml.
Reesults
Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/ml (100 μl/well) can bind biotinylated human CTSL1 with a linear range of 3.2 - 500 ng/ml.
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Synonym
CD74, DHLAG, HLADG, II, Ia-GAMMA, p33, CLIP, INVG34
Other name
CD74, DHLAG, HLADG, II, Ia-GAMMA, p33, CLIP, INVG34
Notes
For Research Use Only! Not to be used in humans
Before use
Centrifuge the vial prior to opening.
Additional source
Recombinants or rec. proteins
Tested applications
SDS-PAGE, N/A
Physical appearance
Lyophilized
